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Kinetic study of anti-viral ribavirin uptake mediated by hCNT3 and hENT1 in Xenopus laevis oocytes
http://hdl.handle.net/10445/4392
http://hdl.handle.net/10445/439219d5311e-ef19-4a95-8d9f-e14a03f7455c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Rivav20091225BioPhysChemRevised.pdf (406.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2010-11-15 | |||||
| タイトル | ||||||
| タイトル | Kinetic study of anti-viral ribavirin uptake mediated by hCNT3 and hENT1 in Xenopus laevis oocytes | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Yamamoto, T.
× Yamamoto, T.× Mitsuru, S.× Takashi, K.× Seiji, M.× Yamaguchi, M.× Tero, A.× Takagi, S.× 中垣, 俊之 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Transport across the cell membrane is crucial in drug delivery. However, the process is complicated because nucleoside derivatives that are commonly used as anti-viral drugs are transported through two different types of specific transporters: concentrative transporters and equilibrative transporters. Cross-disciplinary approaches involving both biological experiments and theoretical considerations are therefore necessary to study the transport of nucleoside analogues such as ribavirin. Here we constructed an experimental model system using the Xenopus laevis oocyte that expressed examples of both types of transporters: human concentrative nucleoside transporter 3 and human equilibrative transporter 1. We also performed a kinetic study. Experimental results showed that the transport of ribavirin could be reduced by inhibiting one of the two types of transporters, which seems to be counterintuitive. We therefore designed a simple mathematical model of the dynamics of ribavirin uptake and analyzed the model behaviors using a numerical simulation. The theoretical results reproduced the experimentally observed phenomena and suggested a possible mechanism for the process. Based on this mechanism, we predicted some potential methods for the effective uptake of ribavirin from a dynamics point of view. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 査読付原著論文 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | インパクトファクター(2.20) | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 被引用回数(1) | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.journals.elsevier.com/biophysical-chemistry/ | |||||
| 書誌情報 |
Biophysical Chemistry 巻 147, p. 59-65, 発行日 2010 |
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| 査読有無 | ||||||
| 値 | あり/yes | |||||
| 研究業績種別 | ||||||
| 値 | 原著論文/Original Paper | |||||
| 単著共著 | ||||||
| 値 | 共著/joint | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.bpc.2009.12.012 | |||||
| 権利 | ||||||
| 権利情報 | copyright©2010 Elsevier.http://www.journals.elsevier.com/biophysical-chemistry/ | |||||
